Intravital deep-tumor single-beam 3-photon, 4-photon, and harmonic microscopy
Three-photon excitation has recently been demonstrated as an effective method to perform intravital microscopy in deep, previously inaccessible regions of the mouse brain.The applicability of 3-photon excitation for deep imaging of other, more heterogeneous tissue types has been much less explored.In this work, we analyze the benefit of high-pulse-